Cortisol, 60 minutes post-injection measures the level of cortisol in the blood one hour after an injection of synthetic ACTH (Cosyntropin) during the ACTH stimulation test. This measurement helps evaluate how well the adrenal glands sustain cortisol production in response to hormonal stimulation over time.

A normal cortisol response at 60 minutes is typically defined as a level of 18–20 µg/dL or higher, although exact thresholds may vary depending on the laboratory and the specific assay method used (for example, immunoassay vs. LC-MS/MS). In healthy individuals, cortisol levels often continue to rise between 30 and 60 minutes, with median values generally ranging from 20 to 30 µg/dL.

A cortisol level below 18 µg/dL at 60 minutes may indicate adrenal insufficiency—either primary (due to adrenal gland dysfunction, such as Addison’s disease) or secondary (due to pituitary disorders or recent corticosteroid use). Some individuals may show a delayed but ultimately adequate cortisol response that appears only at the 60-minute mark.

It is important to interpret the 60-minute cortisol value in conjunction with baseline and 30-minute results, as well as the patient’s clinical presentation. This comprehensive approach provides a more accurate assessment of adrenal function and helps guide further diagnostic or treatment decisions.

Cortisol, the main glucocorticoid (representing 75%-95% of the plasma corticoids), plays a critical role in glucose metabolism and in the body's response to stress.

The majority of cortisol circulates bound to corticosteroid-binding globulin (CBG) and albumin. Normally, less than 5% of circulating cortisol is free (unbound). Only free cortisol can access the enzyme transporters in liver, kidney, and other tissues that mediate metabolic and excretory clearance.

Both hypercortisolism (Cushing disease) and hypocortisolism (Addison disease) can cause disease. 

Cortisol is a steroid hormone synthesized from cholesterol by a multienzyme cascade in the adrenal glands. It is the main glucocorticoid in humans and acts as a gene transcription factor influencing a multitude of cellular responses in virtually all tissues. Cortisol plays a critical role in glucose metabolism, maintenance of vascular tone, immune response regulation, and in the body's response to stress. Its production is under hypothalamic-pituitary feedback control

What is cortisol?

Cortisol is a crucial steroid hormone produced by the adrenal glands, involved in various vital functions including the regulation of metabolism, blood sugar levels, anti-inflammatory actions, and the body's response to stress. To gauge an individual's physiological stress levels or diurnal rhythm, saliva is often used as a non-invasive medium for measuring cortisol.

What is LC/MS?

The liquid chromatography-mass spectrometry (LC/MS) method is a preferred analytical technique for these assessments due to its high specificity and sensitivity in detecting and quantifying cortisol in saliva.

What is cortisol?

Cortisol is a crucial steroid hormone produced by the adrenal glands, involved in various vital functions including the regulation of metabolism, blood sugar levels, anti-inflammatory actions, and the body's response to stress. To gauge an individual's physiological stress levels or diurnal rhythm, saliva is often used as a non-invasive medium for measuring cortisol.

What is LC/MS?

The liquid chromatography-mass spectrometry (LC/MS) method is a preferred analytical technique for these assessments due to its high specificity and sensitivity in detecting and quantifying cortisol in saliva.

What is cortisol?

Cortisol is a crucial steroid hormone produced by the adrenal glands, involved in various vital functions including the regulation of metabolism, blood sugar levels, anti-inflammatory actions, and the body's response to stress. To gauge an individual's physiological stress levels or diurnal rhythm, saliva is often used as a non-invasive medium for measuring cortisol.

What is LC/MS?

The liquid chromatography-mass spectrometry (LC/MS) method is a preferred analytical technique for these assessments due to its high specificity and sensitivity in detecting and quantifying cortisol in saliva.

Cortisol is a stress hormone produced by the adrenal glands and is the primary agent used in our body’s flight or fight response to threatening stimuli. Levels naturally peak in the morning and then reach their lowest point at night. A high blood cortisol level at night may indicate a problem with the adrenal glands; however, individuals who work at night and sleep during the day will have an inversed pattern.

Corticosteroid-binding globulin (CBG), also known as transcortin, is a key plasma protein that plays a critical role in the transport and regulation of corticosteroids in the bloodstream. Produced primarily by the liver, CBG binds to glucocorticoids such as cortisol, the body’s primary stress hormone. By binding these hormones, CBG regulates their availability and helps control their biological activity. Around 80-90% of circulating cortisol is bound to CBG, ensuring that only a small fraction is free and biologically active. This intricate regulation is crucial for maintaining hormone balance and responding to stress.

CBG is not only responsible for cortisol transport, but it also acts as a reservoir that releases cortisol in response to changing physiological needs. When the body encounters stress, injury, or inflammation, enzymes like neutrophil elastase can cleave CBG, releasing bound cortisol to target tissues. This enables a rapid response to acute stress, increasing the body’s ability to cope with immediate physiological challenges. Without this mechanism, the body would struggle to meet the hormonal demands necessary to maintain homeostasis in stressful situations.

Cortisol / cortisone ratio indicates activity of HSD11B2 activity and assessment of tissue specific concentrations of cortisol, which normally cannot be measured without a biopsy.

Cortisol is a steroid hormone synthesized from cholesterol by a multienzyme cascade in the adrenal glands. It is the main glucocorticoid in humans and acts as a gene transcription factor influencing a multitude of cellular responses in virtually all tissues. Cortisol plays a critical role in glucose metabolism, maintenance of vascular tone, immune response regulation, and in the body's response to stress. Its production is under hypothalamic-pituitary feedback control.

Cortisone, a downstream metabolite of cortisol, provides an additional variable to assist in the diagnosis of various adrenal disorders, including abnormalities of 11-beta-hydroxy steroid dehydrogenase (11-beta HSD), the enzyme that converts cortisol to cortisone. Deficiency of 11-beta HSD results in a state of mineralocorticoid excess because cortisol (but not cortisone) acts as a mineralocorticoid receptor agonist. Licorice (active component glycyrrhetinic acid) inhibits 11-beta HSD and excess consumption can result in similar changes.

Cortisol / cortisone ratio indicates activity of HSD11B2 activity and assessment of tissue specific concentrations of cortisol, which normally cannot be measured without a biopsy.

Cortisol is the main glucocorticoid released from the adrenal gland in response to stress. High levels of cortisol have been reported in cases of Cushing’s disease, malnutrition, early life stress, hypothyroidism, depression, alcoholism, obesity, and critical illness. Additionally, exogenous exposure to glucocorticoids prior to testing may be a source of cortisol elevations.

The Cortisol/day measurement on a HUMAP panel is a crucial indicator of cortisol levels throughout a typical day, reflecting the hormone's diurnal pattern and its overall balance in the body. Cortisol, produced by the adrenal glands, is integral to many bodily functions, such as regulating metabolism, immune response, and the body's stress response. Normally, cortisol levels exhibit a diurnal rhythm: they are highest in the early morning, aiding in waking up and providing energy for the day ahead, and gradually decrease as the day progresses, reaching their lowest levels late in the evening and during early night. This pattern ensures optimal functioning of various physiological processes, including the sleep-wake cycle and blood sugar regulation.

The Cortisol/DHEA-Sulfate (DHEAS) Ratio in the context of the Cortisol Awakening Response (CAR) represents a crucial biomarker in psychoneuroendocrinology, providing valuable insights into the dynamic interplay between the hypothalamic-pituitary-adrenal (HPA) axis and adrenal steroidogenesis under conditions of stress and physiological arousal.

Cortisol, a glucocorticoid hormone, plays a pivotal role in the body's response to stress, influencing various physiological processes, including metabolism, immune function, and circadian rhythm regulation.

DHEAS, an adrenal androgen, serves as a counter-regulatory hormone, often associated with anabolic functions and resilience against stress.