Estrogens and Metabolites (Urine) - DUTCH
Urine
Performed by: Precision Analytical
Estrogen’s basic biological function is as an anabolic hormone. There are three primary forms of estrogen: estrone (E1); estradiol (E2); and estriol (E3). All three forms have varying levels of activity and specificity for different tissues. E2 is the most active of the estrogens and is responsible for most of the actions attributed to the estrogens.
Where is estrogen made?
Estrogen is made primarily in the ovaries of cycling women while smaller amounts can be converted from testosterone through a process called aromatization. In menopause estrogen production shifts almost completely to aromatization. The enzyme responsible for aromatization is found in fat tissue, the brain, gonads, blood vessels, skin, and bone.
In Men:
Men need far less estrogen than women and it is aromatized primarily from testosterone. Women who are still cycling have much greater quantities of estradiol than men, but it is important to note that men do make estrogen. In fact, the level of estrogen in men is actually higher than the level in a post-menopausal woman!
What does estrogen do?
- Growth and development of female secondary sex characteristics
- Thickens the uterus for implantation, increases vaginal acidity to protect against infection, important for vaginal lubrication (particularly E3)
- Necessary for bone health
- In the brain helps maintain body temperature, protects against memory loss, increases serotonin and serotonin receptors
- Important for collagen production, skin thickness and getting blood supply to the skin
- Helps protect against atherosclerosis
What is a metabolite?
When the body makes a hormone such as estrogen it must be processed by the body in the liver through the process of phase 1 and phase 2 detoxification before it can be excreted out the other end. This is known as hormone metabolism. As a hormone is undergoing that process, it gets changed into other forms known as metabolites.
Phase 1 metabolism:
In the liver, estrone and estradiol are converted into the phase 1 metabolites known as 2-OH-E1, 4-OH-E1 or 16-OH-E1. These metabolites can go back out into circulation before going through phase 2 detoxification where they are then excreted out of the body. The phase 1 metabolites can be more damaging to the body by causing estrogen- dominant symptoms or increasing the risk for cancer in both men and women.
Generally speaking, the 2-OH-E1 is considered the safer metabolite as it only weakly induces cell proliferation and might even inhibit it, the 4-OH-E1 can damage DNA and may be more cancer inducing.
You can assess phase 1 metabolism by looking at the relative amounts of these hormones.
The normal estrogen metabolism ratio of the three (2-OH-E1 + 4OH-E1 + 16OH-E1) is as follows:
- 70% of the total usually makes up the 2-OH-E1 fraction
- 10% comes from the 4-OH-E1
- 20% from 16-OH-E1
Phase 2 metabolism (methylation):
After estrogens go through phase 1 detoxification, they move on to methylation which is part of phase 2 detoxification. Methylation, in essence, “neutralizes” the phase 1 metabolites and prepares them to be excreted out of the body instead of shuttling them back into circulation. Only 2-OH and 4-OH estrogens are methylated. 16-OH-E1 is not.
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Biomarkers included in this panel:
16α-Hydroxyestrone (16α-OH-E1), or hydroxyestrone, also known as estra-1,3,5(10)-trien-3,16α-diol-17-one, is an endogenous steroidal estrogen and a major metabolite of estrone, as well as an intermediate in the biosynthesis of estri
Learn more16α-Hydroxyestrone (16α-OH-E1), or hydroxyestrone, also known as estra-1,3,5(10)-trien-3,16α-diol-17-one, is an endogenous steroidal estrogen and a major metabolite of estrone, as well as an intermediate in the biosynthesis of estri
Learn moreThe 2-Methoxy Estrogens are considered to be protective. 2-Methoxy-E1 is produced from 2-OH-E1 through the COMT enzyme. Anti-cancerogenic effects have been ascribed to 2-OH-E1 and particularly 2-Methoxy-E1. 2-Methoxy-E1 has shown antiproliferat
Learn moreThe 2-Methoxy Estrogens are considered to be protective. 2-Methoxy-E1 is produced from 2-OH-E1 through the COMT enzyme. Anti-cancerogenic effects have been ascribed to 2-OH-E1 and particularly 2-Methoxy-E1. 2-Methoxy-E1 has shown antiproliferat
Learn moreThe 2-Methoxy Estrogens are considered to be protective. 2-Methoxy estrogens are produced from 2-OH estrogens through the COMT enzyme. Anti-cancerogenic effects have been ascribed to 2-OH estrogens and particularly 2-Methoxy estroge
Learn moreThe 2-Methoxy Estrogens are considered to be protective. 2-Methoxy estrogens are produced from 2-OH estrogens through the COMT enzyme. Anti-cancerogenic effects have been ascribed to 2-OH estrogens and particularly 2-Methoxy estroge
Learn moreMost consider 2-OH-E1 favorable Estrogen is metabolized (primarily by the liver) down three phase I pathways. The 2-OH pathway is considered the safest because of the anti-cancer properties of 2-OH metabolites. Conversely, the 4-OH pathway is cons
Learn moreMost consider 2-OH-E1 favorable Estrogen is metabolized (primarily by the liver) down three phase I pathways. The 2-OH pathway is considered the safest because of the anti-cancer properties of 2-OH metabolites. Conversely, the 4-OH pathway is cons
Learn moreResearch and clinical studies show that the 2-hydroxylated estrogens (2-OH E2 and 2-OH E1) are a safer pathway of hydroxylation than the 4-hydroxyestrogens (4-OH E2 and 4-OH E1), which bind to and damage DNA, leading to mutations that are associated
Learn moreResearch and clinical studies show that the 2-hydroxylated estrogens (2-OH E2 and 2-OH E1) are a safer pathway of hydroxylation than the 4-hydroxyestrogens (4-OH E2 and 4-OH E1), which bind to and damage DNA, leading to mutations that are associated
Learn more- The 4-OH pathway is considered the most genotoxic as its metabolites can create reactive products that damage DNA. - Estrone is hydroxylated through Phase 1 detox to form 4-OH-E1. In Phase 2 it is methylation to form 4-MeE1. When 4-OH-E1 is prop
Learn more- The 4-OH pathway is considered the most genotoxic as its metabolites can create reactive products that damage DNA. - Estrone is hydroxylated through Phase 1 detox to form 4-OH-E1. In Phase 2 it is methylation to form 4-MeE1. When 4-OH-E1 is prop
Learn more4 hydroxy estrone (4-OH-E1) and estradiol (4-OH-E2) are metabolites of estrone and estradiol and are very reactive estrogens. They are highly prone to the formation of catechol estrogen-derived 3,4 semi-quinones, which are potent, electrophilic,
Learn more4 hydroxy estrone (4-OH-E1) and estradiol (4-OH-E2) are metabolites of estrone and estradiol and are very reactive estrogens. They are highly prone to the formation of catechol estrogen-derived 3,4 semi-quinones, which are potent, electrophilic,
Learn moreThe most physiologically active estrogen. Binds to both to ER and Estrogen Receptor Beta (ERβ). Estradiol, made in the ovary, rapidly converts to estrone. Poor symptom control with estrogen replacement may suggest the need for improving abs
Learn moreThe most physiologically active estrogen. Binds to both to ER and Estrogen Receptor Beta (ERβ). Estradiol, made in the ovary, rapidly converts to estrone. Poor symptom control with estrogen replacement may suggest the need for improving abs
Learn moreHas weak estrogen activity. Considered to be a protective estrogen. Most prevalent estrogen in pregnancy.
Learn moreHas weak estrogen activity. Considered to be a protective estrogen. Most prevalent estrogen in pregnancy.
Learn moreProduced by the ovaries, the estrone hormone is one of three types of estrogen, and it is one of the major hormones found in the bodies of postmenopausal women. While research into estrone function is still ongoing, since it is the least powerful of
Learn moreProduced by the ovaries, the estrone hormone is one of three types of estrogen, and it is one of the major hormones found in the bodies of postmenopausal women. While research into estrone function is still ongoing, largely due to the fact that it is
Learn moreTotal estrogen is a reliable test for estrogen status and is used to detect hormone imbalances. Estrogen is known as the “female” hormone. The four major naturally occurring estrogens in women are estrone (E1), estradiol
Learn moreTotal estrogen is a reliable test for estrogen status and is used to detect hormone imbalances. Estrogen is known as the “female” hormone. The four major naturally occurring estrogens in women are estrone (E1), estradiol
Learn more